Welcome to Laboratory of Biophysical Chemistry

Drug Design at the single molecule level..

Our Research Work

Our aim is to develop rational methodologies for drug discovery..
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Drug Design

Medicinal Chemistry

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Protein Dynamics


Human 15-Lipoxygenase-1

Lipoxygenases (LOXs) catalyze the insertion of oxygen into polyunsaturated fatty acids, resulting in signaling compounds that regulate several inflammatory diseases. Our research focuses on human 15-lipoxygenase-1 (15-LOX-1), an enzyme which is widely expressed in the central nervous system (CNS), and its catalysis products are critical factors in the emergence of brain pathology. There is a solid proof of evidence that connects 15-LOX-1 with various CNS diseases like stoke as well as Alzheimer’s and Parkinson’s diseases. In the light of the above information, we aim to thorough investigate 15-LOX-1 dynamics and develop next generation drug-like inhibitors. 


More than a third of cellular proteins are exported from the cytoplasm, where they are synthesized, into and across cellular membranes. Sec pathway export is an essential process that takes proteins like antibodies and toxins, across the eukaryotic ER or prokaryotic plasma membranes. Post-translational translocation is used by the ~500 secretory preproteins that fully cross the inner membrane of E.coli through SecYEG and are released at the trans periplasmic side. SecA is the primary translocase receptor recognizing the preproteins. Our aim is to successfully define the SecA dynamics and to develop small molecule modulators as potential antibiotics. 

A Little About Us

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Dr. Nikolaos Eleftheriadis
Assistant Professor
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Giorgos Kanakarakis
PhD student
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Ntaniela Spacho
MSc student
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Emmanouil Giotas
MSc student
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Christos Papadopoulos
MSc student
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Antonis Georgakopoulos
BSc student
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Areti Karamitrou
BSc student

Selected Publications

  • Zhang, L.; Isselstein, M.; Köhler, J.; Eleftheriadis, N.; Huisjes, N.M.; Guirao-Ortiz, M.; Narducci, A.; Smit, J.H.; Stoffels, J.; Harz, H.; Leonhardt, H.; Herrmann, A.; Cordes, T. Linker Molecules Convert Commercial Fluorophores into Tailored Functional Probes during Bio-labeling. Angew. Chemie Int. Ed. 2022, e202112959
  • Krishnamurthy, S.; Sardis, M.F.; Eleftheriadis, N.; Chatzi, K.E.; Smit, J; Karathanou, K.; Gouridis, G.; Portaliou, A; Bondar, A.N; Karamanou, S; Economou A. Preproteins couple the intrinsic dynamics of SecA to its ATPase cycle to translocate via a catch and release mechanism. Cell Reports 2022, 38 (6), 110346
  • Eleftheriadis, N.; Krishnamurthy, S.; Karathanou, K.; Smit, J; Portaliou, A; Chatzi, K.E.; Karamanou, S; Bondar, A.N; Gouridis, G.; Economou A. A nexus of intrinsic dynamics underlies translocase priming Structure 2021, 29, 1-13
  • de Boer, M.; Gouridis, G.; Vietrov, R.; Begg, S. L.; Schuurman-Wolters. G.K.; Husada, F.; Eleftheriadis, N.; Poolman, B.; McDevitt, C. A.; Cordes, T. Conformational and dynamic plasticity in substrate-binding proteins underlies selective transport in ABC importers. E-life, 2019, 8, e44652
  • Eleftheriadis, N.; Wojcik, M.; Zwinderman, M. R. H.; Dömling, A.; Dekker, F. J.; Boersma Y. Identification of potential antivirulence agents by substitution-oriented screening for inhibitors of Streptococcus pyogenes Sortase A. Eur. J. Med. Chem. 2019, 161, 93–100
  • Eleftheriadis, N.; Thee, S. A.; Zwinderman, M. R. H.; Leus, N. G. J.; Dekker, F. J. Activity‐Based Probes for 15‐Lipoxygenase‐1. Angew. Chemie Int. Ed. 2016, 55, 12300 –12305
  • Eleftheriadis, N.; Poelman, H.; Leus, N. G. J.; Honrath, B.; Neochoritis, C. G.; Dolga, A.; Dömling, A.; Dekker, F. J. Design of a Novel Thiophene Inhibitor of 15-Lipoxygenase-1 with Both Anti-Inflammatory and Neuroprotective Properties. Eur. J. Med. Chem. 2016, 122, 786–801


Read our latest news
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Feb, 2022
Molecular Modeling!

We are happy to announce that our Molecular Docking and Cheminformatics softwares are ready! Are you ready for new findings? 

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April, 2022
Finally, we did it!

Yes, it is true.. We finally did it. We have our own group website! Here, you can find detailed information about our exciting projects and methodologies.

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May, 2022
Microscope is loading..

We are almost there...

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Nov, 2022
Our Microscope is ready!

Our state-of-the-art Confocal Fluorescence Microscope is ready! Do you think that is impossible to study protein dynamics at the single molecule level? Think again!

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