Welcome to Laboratory of Biophysical Chemistry

Drug Design at the single molecule level..

Our Research Work

Our aim is to develop rational methodologies for drug discovery..
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Drug Design

Medicinal Chemistry

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Protein Dynamics

smFRET

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ChessBioChem

Checkmate to your protein..

Human 15-Lipoxygenase-1

Lipoxygenases (LOXs) catalyze the insertion of oxygen into polyunsaturated fatty acids, resulting in signaling compounds that regulate several inflammatory diseases. Our research focuses on human 15-lipoxygenase-1 (15-LOX-1), an enzyme which is widely expressed in the central nervous system (CNS), and its catalysis products are critical factors in the emergence of brain pathology. There is a solid proof of evidence that connects 15-LOX-1 with various CNS diseases like stoke as well as Alzheimer’s and Parkinson’s diseases. In the light of the above information, we aim to thorough investigate 15-LOX-1 dynamics and develop next generation drug-like inhibitors. 

SecA

More than a third of cellular proteins are exported from the cytoplasm, where they are synthesized, into and across cellular membranes. Sec pathway export is an essential process that takes proteins like antibodies and toxins, across the eukaryotic ER or prokaryotic plasma membranes. Post-translational translocation is used by the ~500 secretory preproteins that fully cross the inner membrane of E.coli through SecYEG and are released at the trans periplasmic side. SecA is the primary translocase receptor recognizing the preproteins. Our aim is to successfully define the SecA dynamics and to develop small molecule modulators as potential antibiotics. 

A Little About Us

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Christos Papadopoulos
MSc student
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Eirini-Eleni Kalaitzaki
MSc student
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Ioannis Angelonidis
MSc student
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Stavroula-Aikaterini Kaplani
MSc student
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Marina Somaraki
MSc student
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Thanos Panousis
MSc student
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Olga-Maria Papaggeli
MSc student
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Maritina Giannakaki
MSc student
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Vasilina Souropani
MSc student
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Dr. Anastasia Louka
Postdoctoral researcher
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Dr. Nikolaos Eleftheriadis
Assistant Professor

Selected Publications

  • Louka, A.; Spacho, N.; Korovesis, D.; Adamis, K.; Papadopoulos, C.; Kalaitzaki, E.E.; Tavernarakis, N.; Neochoritis, C.; Eleftheriadis, N.* Crafting Molecular Tools for 15-Lipoxygenase-1 in a Single Step. Angew. Chem. Int. Ed. 2024, e202418291
  • Spacho, Ν.; Casertano, Μ.; Imperatore, C.; Papadopoulos, C.; Menna, M;* Eleftheriadis, N.* Investigating the Catalytic Site of Human 15-Lipoxygenase- 1 via Marine Natural Products. Chemistry—A European Journal, 2024, e202402279
  • Giotas, E.; Kaplani, S.A.; Eleftheriadis, N.* The Multifunctional Preprotein Binding Domain of SecA. ChemBioChem 2024, e202400621
  • Zhang, L.; Isselstein, M.; Köhler, J.; Eleftheriadis, N.; Huisjes, N.M.; Guirao-Ortiz, M.; Narducci, A.; Smit, J.H.; Stoffels, J.; Harz, H.; Leonhardt, H.; Herrmann, A.; Cordes, T. Linker Molecules Convert Commercial Fluorophores into Tailored Functional Probes during Bio-labeling. Angew. Chemie Int. Ed. 2022, e202112959
  • Krishnamurthy, S.; Sardis, M.F.; Eleftheriadis, N.; Chatzi, K.E.; Smit, J; Karathanou, K.; Gouridis, G.; Portaliou, A; Bondar, A.N; Karamanou, S; Economou A. Preproteins couple the intrinsic dynamics of SecA to its ATPase cycle to translocate via a catch and release mechanism. Cell Reports 2022, 38 (6), 110346
  • Eleftheriadis, N.; Krishnamurthy, S.; Karathanou, K.; Smit, J; Portaliou, A; Chatzi, K.E.; Karamanou, S; Bondar, A.N; Gouridis, G.; Economou A. A nexus of intrinsic dynamics underlies translocase priming Structure 2021, 29, 1-13
  • de Boer, M.; Gouridis, G.; Vietrov, R.; Begg, S. L.; Schuurman-Wolters. G.K.; Husada, F.; Eleftheriadis, N.; Poolman, B.; McDevitt, C. A.; Cordes, T. Conformational and dynamic plasticity in substrate-binding proteins underlies selective transport in ABC importers. E-life, 2019, 8, e44652
  • Eleftheriadis, N.; Wojcik, M.; Zwinderman, M. R. H.; Dömling, A.; Dekker, F. J.; Boersma Y. Identification of potential antivirulence agents by substitution-oriented screening for inhibitors of Streptococcus pyogenes Sortase A. Eur. J. Med. Chem. 2019, 161, 93–100
  • Eleftheriadis, N.; Thee, S. A.; Zwinderman, M. R. H.; Leus, N. G. J.; Dekker, F. J. Activity‐Based Probes for 15‐Lipoxygenase‐1. Angew. Chemie Int. Ed. 2016, 55, 12300 –12305
  • Eleftheriadis, N.; Poelman, H.; Leus, N. G. J.; Honrath, B.; Neochoritis, C. G.; Dolga, A.; Dömling, A.; Dekker, F. J. Design of a Novel Thiophene Inhibitor of 15-Lipoxygenase-1 with Both Anti-Inflammatory and Neuroprotective Properties. Eur. J. Med. Chem. 2016, 122, 786–801

News/Events

Read our latest news
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Feb, 2022
Molecular Modeling!

We are happy to announce that our Molecular Docking and Cheminformatics softwares are ready! Are you ready for new findings? 

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April, 2022
Finally, we did it!

Yes, it is true.. We finally did it. We have our own group website! Here, you can find detailed information about our exciting projects and methodologies.

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Nov, 2022
Our Microscope is ready!

Our state-of-the-art Confocal Fluorescence Microscope is ready! Do you think that is impossible to study protein dynamics at the single molecule level? Think again!

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2024
New papers!

Hard work truly pays off!
We are excited to share our latest articles:
 https://doi.org/10.1002/chem.202402279
https://doi.org/10.1002/cbic.202400621
https://doi.org/10.1002/anie.202418291

Contact Us

Phone

+30 2810545053

Email

n.eleftheriadis@uoc.gr 

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